Figure 3.
(A and D) ATR-IR spectra of plain SSMs (⋯··) consisting of DOPC/MCCDOPE 90:10 in D2O with 50 mM NaCl spread on an Si-covered ZnSe crystal. Lipid bands and the amide I region are shown. LPs were formed with K3Cys (A) or i-K3Cys (D) covalently attached to MCCDOPE (- - -). Spectra obtained after coiled-coil formation with E3Cys (A) or i-E3Cys (D) are also shown (—). (B and E) Time course of LP coupling reaction followed by coiled-coil formation shown in A and D, respectively. The intensity of the amide I band is plotted versus time. The time course starts after bilayer formation (0 min). Addition of peptides and rinsing is indicated by arrows. Panel C shows the deconvoluted amide I band for the coiled-coil build of LP-K3Cys + E3Cys. In panel F deconvoluted amide I band of LP-i-K3Cys + i-E3Cys is shown. (C and F) Single Gaussian fits are shown in black, and the amide I band of the spectra is in gray. Black scattered lines represent the sum of all Gaussian fits.