Table 1.

Secondary structure of peptides before and after formation of coiled-coil dimers, obtained from CD measurements in solution (c_peptide = 0.1 mM; in phosphate buffer (50 mM Na2HPO4, pH 6.8) PB 6.8)

	E3Cys	K3Cys	i-E3Cys	i-K3Cys	E3Cys + K3Cys	i-E3Cys + i-K3Cys	i-E3Cys + K3Cys	E3Cys + i-K3Cys
α-helix/ %	31	46	31	44	79	78	73	79
β-sheet/ %	12	23	12	23	0	0	2	0
random/ %	57	31	57	33	21	21	24	21
[θ]220/[θ]208	0.69	0.87	0.71	0.75	1.00	0.98	0.94	1.03

Fits were obtained with DichroWeb online analysis software (18,19).