Table 2.
Dissociation constants KD and corresponding free enthalpies ΔG° for coiled-coil formation determined by CD spectroscopy and SPR, and at the membrane interface by ellipsometry (Langmuir)
| Method | K3Cys + E3Cys |
i-K3Cys + i-E3Cys |
K3Cys + i-E3Cys |
i-K3Cys + E3Cys |
||||
|---|---|---|---|---|---|---|---|---|
| KD/μM | ΔG°/kBT | KD/μM | ΔG°/kBT | KD/μM | ΔG°/kBT | KD/μM | ΔG°/kBT | |
| CD | 4.1 ± 0.7 | −12.4 | 7.5 ± 0.6 | −11.8 | 2.9 ± 0.8 | −12.8 | 2.5 ± 0.6 | −12.9 |
| SPR | 0.5 ± 0.3 | −14.5 | 2.3 ± 1.8 | −13.0 | 0.5 ± 0.3 | −14.5 | 1.2 ± 1.0 | −13.6 |
| Langmuir | 28 ± 1 | −10.5 | 25 ± 3 | −10.6 | 31 ± 1∗ | −10.4 | 28 ± 2 | −10.5 |
∗
Ellipsometry data were fitted with the Bragg-Williams isotherm.