Table 1.

Thermodynamic and affinity changes for Cdc13-DBD binding to Tel-11 containing a degenerate library substitution at each site in the DNA.

Sample	Sequence	ΔΔG (kcal/mol) ensemble	Average relative Kd
WT	GTGTGGGTGTG		1.0
1		2.5 ± 0.3	95
2		1.0 ± 0.1	5.9
3		3.4 ± 0.1	450
4		3.2 ± 0.3	360
5		−0.6 ± 0.1	0.4
6		0.1 ± 0.1	1.1
7		0.9 ± 0.1	5.0
8		−0.5 ± 0.2	0.4
9		0.8 ± 0.2	4.1
10		0.2 ± 0.2	1.4
11		−0.1 ± 0.02	0.9