Table 2.

Thermodynamic and affinity changes for Cdc13-DBD binding to Tel-11 containing single substitutions.

Sample	Sequence	Average ΔΔG (kcal/mol)	Average relative Kd
WT	GTGTGGGTGTG	0	1
A1	ATGTGGGTGTG	2.9 ± 0.1	200
C1	CTGTGGGTGTG	3.3 ± 0.1	370
T1	TTGTGGGTGTG	2.8 ± 0.1	170
S1	STGTGGGTGTG	2.8 ± 0.2	160
I1	ITGTGGGTGTG	0.7 ± 0.1	3.8
A3	GTATGGGTGTG	3.3 ± 0.1	410
C3	GTCTGGGTGTG	4.3 ± 0.1	>2300
T3	GTTTGGGTGTG	3.5 ± 0.4	>540
S3	GTSTGGGTGTG	3.2 ± 0.4	340
I3	GTITGGGTGTG	0.2 ± 0.1	1.4
A4	GTGAGGGTGTG	3.9 ± 0.1	>1100
C4	GTGCGGGTGTG	3.7 ± 0.1	>830
G4	GTGGGGGTGTG	2.8 ± 0.1	170
U4	GTGUGGGTGTG	0.4 ± 0.1	2.2