Figure 4.

Association (0–360 s) and dissociation (360–600 s) kinetics for anti-IL-6 at two different surface coverages in PBS-T containing 0.1% BSA at a flow rate of 30 μL min⁻¹ showing SPR responses for a series of total antibody concentrations (a- 6.4 nM, b- 9.6 nM, c-21.2 nM, d- 41.1 nM, e- 76.1 nM) in PBS-T containing 0.1% BSA at 30 μL min⁻¹ for (A) immobilized IL-6 surface density of 6.0 × 10⁹ molecules mm⁻² and (B) IL-6 surface density of 1.1 × 10¹⁰ molecules mm⁻²