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Illustration of the experimental plan in this paper. Multivalent co-operative interactions of antibody-protein on magnetic particles at different surface densities of the antigen protein are illustrated for PSA and IL-6. (Antigen surface densities were calculated as follows: 1 micro Refractive Index Unit (μRIU) = 0.73 Response Unit (RU), (Reichert, Inc., NY) and 1 RU corresponds to 1 pg mm−2 of protein antigen immobilized on surface)
