Table 1.

Binding parameters for free antibodies with proteins at different surface densities on Au surfaces from SPR

Interaction	surf. den. molec. mm−2	ka(M−1s−1)/104	kd(s−1)/10−4	KD, (nM)	Ref./Instr.
PSA binding Ab1	7.7 × 109	37.0 ± 0.3	11.1 ± 0.2	3.0 ± 0.2	This work
PSA binding Ab1	2–8.0 × 109	4.1± 0.6	0.4 ± 0.1	1.1 ± 0.2	23, Biacore
PSA binding Ab2 Figure 1	9.0 × 109	10 ± 1	3.3 ± 0.2	3.1 ± 0.2	This work
	2.7 × 109	7.9 ± 0.1	3.2 ± 0.1	4.0 ± 0.1
IL-6 binding Ab1	1.1 × 1010	15.0 ± 0.2	12.0 ± 0.3	8.0 ± 0.2	This work
IL-6 binding Ab1	6.1 × 109	3.72	2.93	7.9	25, ProteOn
IL-6 binding Ab2 Figure 2	2.1 × 1010	7.6 ± 0.1	4.8 ± 0.2	6.3 ± 0.3	This work
	2.5 × 109	3.9 ± 0.1	3.1 ± 0.2	8.0 ±0.5

Abbreviations: surf. den. molec. – Surface density of molecules; Ref./Instr. – References/Instruments