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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: Pflugers Arch. 2012 Oct 7;464(6):583–592. doi: 10.1007/s00424-012-1167-y

Figure 4.

Figure 4

A: Example of overlapping immunostaining image for Von willebrand Factor (green arrow), α-actin (red arrow), Dapi (blue arrow), and over-lap between Von willebrand and α-actin (yellow arrow) in mouse hind-limb; B: Amplification of a portion from image A; C: Effect of sodium nitrite (SN) treatment on the capillary density in sham and hypertensive mice induced by chronic infusion of angiotensin II (Ang II) or L-NAME. Capillary density was assessed by von Willebrand immunostaining, n=5; *P < 0.05 for sham vs. L-NAME treatment and Ang II; #P < 0.05 for sham, L-NAME and Ang II treated with sodium nitrite vs. sham, L-NAME and Ang II.