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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: Urology. 2012 Aug 28;80(6):1391.e1–1391.e7. doi: 10.1016/j.urology.2012.07.008

Figure 2.

Figure 2

Effect of 5-ALA and ALA-HE on the expression of XIAP and sensitization to TRAIL-mediated apoptosis in normal and malignant prostate cell lines. (A) PC-3, DU-145 prostate cancer cells and PZ-HPV-7 normal prostate epithelial cells were cultured in medium alone or in the presence of indicated concentrations of either 5-ALA or ALA-HE for 24 hours. Expression of XIAP and GAPDH was detected by immunoblotting with specific antibodies. Expression of GAPDH was used to document equal protein loading. (B) Cells were cultured in medium alone or in the presence of either 5-ALA or ALA-HE (200µM) with or without TRAIL (200ng/ml) overnight. The percentage of apoptotic cells was determined by TUNEL assay followed by flow cytometry analysis. Numbers represent percentage of apoptotic cells. Representative data provided from one of the three experiments.