Figure 5.

Event number density plots for (A) 0 mM and (B) 100 mM Mg⁺² in TE buffer with 20% glycerol. The insert in Fig. 5B shows three events, one corresponding to a circular DNA and the other two corresponded to branched or bent supercoiled DNA. (C) Left, t_d as a function of the amount of Mg⁺² ions added. Right, the change in ${\text{t}}_{\text{d}}/t_d^0$. The Dashed line is the theoretically predicted values from MCC theory (see SI.4.). (D) Histograms of the number of 5-μs current blockage samples per 2 pA bin for 0 and 100 mM Mg⁺². A ~14 nm pore was used with I₀ = 11.25 nA.