Figure 2.

PCR validation scheme of L1-seq results. (A) The three-step PCR validation scheme and location of primers used. Triangles symbolize TSD. (B) PCR validation of the 3′ junction (ins. 7). This insertion is in tumor 1 of the eight DNA samples that had been pooled for Illumina sequence analysis (left), while the right panel shows it is present exclusively in the tumor, but not in the normal colon. The higher molecular weight band visible above the ins. 7 empty site PCR product in the tumor is a highly truncated L1. (T) Tumor; (N) normal colon; (FS) filled site PCR product; (ES) empty site PCR product.