Figure 4.

In vivo and in vitro human nucleosomes show a similar inversion of correlation trends. (A) Comparison of in vivo– and in vitro–reconstituted nucleosomes in human T-cells (CD4⁺ or SP) or using T-cell-derived genomic DNA and presented as in Figure 3A at all promoters (7021 regions). Whereas in vivo the aNDR appears in subgroup B, it appears in group D in vitro. (B) Correlation of CpG peak length and aNDR size in vitro and in vivo within CGI-rich groups as in Figure 3B. (C) GC content and MNase signal at +1 nucleosome proximity (as in Fig. 3C) from data presented in A. A similar trend of inversion from correlation to anti-correlation is observed between +1 nucleosome density and GC content but with different GC content thresholds.