Figure 5.

Generation of small RNA from the heterochromatic sequences. Sequence reads of small RNA libraries produced from 0 to 1-h embryos, 2 to 6-h embryos, 6 to 10-h embryos, imaginal discs, female bodies, female heads, male bodies, or male heads (Chung et al. 2008) were aligned to the probe sequences, and the number of reads that match each probe was measured. (A) Heterochromatic regions differ in their production of small RNAs corresponding to different types of repetitive sequences. All show overall higher levels of small RNA than that of the euchromatic genes (Eu), but between different heterochromatic regions the overall abundance of small RNAs and the type of repetitive sequences they originate from vary substantially. Inset highlights the log₂(small RNA reads) of the major satellite DNAs at the region. (B) Temporal profile of small RNA expression. Heat map showing log₂ (small RNA reads) at different developmental stages.