Figure 1.

Genetic complement deficiency impairs SV40⁺ ovarian tumor growth and development. (A) Tumors were collected from 16-week-old Tg⁺C3^WT, Tg⁺C3^HET, and Tg⁺C3^KO mice and measured with calipers. Volumes were calculated using the mathematical formula for the volume of an ellipsoid: V = 4/3(3.1416) abc, where a, b, and c represent the semi-axes of each diameter. P < .05. (B) Confocal microscopy of frozen tumor sections labeled with anti-C3b/iC3b/C3c antibody and 4′,6′-diamino-2-phenylindole (DAPI). Original magnification, x23. Results are representative of four Tg⁺C3^WT tumors, four Tg⁺C3^HET tumors, and two Tg⁺C3^KO tumors. (C) Quantification of C3b/iC3b/C3c deposition on tumor cells from 16-week-old Tg⁺C3^WT, Tg⁺C3^HET, and Tg⁺C3^KO mice (n/group = 5). Bars represent means ± standard error. (D) Cell composition of ovarian tumors in Tg⁺C3^WT (n = 3), Tg⁺C3^HET (n = 5), and Tg⁺C3^KO (n = 3) mice by flow cytometry. Tumor cells were identified by staining with anti-SV40 Ab and tumor-infiltrating leukocytes with anti-CD45 Ab.