Figure 3.

Genetic complement deficiency impairs neo-angiogenesis. (A) Tumor microvasculature was evaluated by CD31 staining (dark blue staining) of frozen tumor sections from 16-week-old Tg⁺C3^WT and Tg⁺C3^HET mice. Values reflect the number of CD31⁺ vessels averaged from five high-power fields per slide per mouse. Original magnification, x10. Bars represent means of CD31⁺ microvessels ± standard error. (B-D) Bars represent the absolute number of total cells (B), or CD31⁺ ECs (C), or the ratios of CD31⁺ ECs and total cells (D) attracted to Matrigel plugs admixed with recombinant VEGF (10 ng/ml) and implanted in C57BL/6 mice (WT, gray bar, n/group = 5) or C3^KO mice (black bar, n/group = 5) ± standard error.