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. 2012 Nov;14(11):1087–1096. doi: 10.1593/neo.121342

Figure 4.

Figure 4

Expression of mRNA chimeras in normal tissues. (A) (Left) Agarose electrophoresis analysis of real-time PCR products (Sybr Green real-time PCR). (Right) Real-time PCR analysis of the CHD2-CHMP1A chimeric mRNA in normal tissues (PrimeTime real-time PCR). HBL-100 cell cDNA was used as a positive control. (Bottom) Amplification plots of CHD2-CHMP1A mRNA in the HBL-100 breast cancer cell line (blue) and normal breast tissue (red). CHD2-CHMP1A (top lanes) and GAPDH (bottom lanes) were amplified in duplicate. Green triangles, successful amplification; red triangles, no amplification. (B) Agarose electrophoresis of nested PCR products of PRKAA1-TTC33, SAMM50-PARVB, CTBS-GNG5, and URB1-C21orf45 in normal tissues.