Figure 5.
Human AML cells fuse also with dendritic and endothelial cells. (A) Upper panels show flow cytometry analysis of cells coexpressing human CD45, CD33, and CD14 that express also murine CD11c, a dendritic cell-specific marker. Lower panels show flow cytometry analysis of mouse endothelial cells (Flk-1-positive) expressing human CD45 and human CD31. (B) In addition to AML, ALL cell transplantation induces cell fusion in vivo; 1 x 107 primary human ALL cells were injected ip, and 47 α 6 (ALL-B) or 72 ± 13 (ALL-T) days later, animals were sacrificed and spleen cells were analyzed by flow cytometry for the co-expression of human and mouse CD45. (C) Human CD45 and mouse CD45 flow cytometry expression patterns of BM cells from a primary and a secondary recipient of human cord blood CD34+ cells showing absence of hybrid cells.