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. 2012 Nov;14(11):1032–1042. doi: 10.1593/neo.121368

Figure 6.

Figure 6

IHC against cell cycle markers and EGFR protein for DU145 and LNCaP tumor xenografts. (A and B) Ki67, (C and D) p27KIP, and (E and F) EGFR IHC quantifications relative to respective NT control in both DU145-and LNCaP-derived tumors. Ki67 proliferation index was determined by counting cells with positive nuclear staining in the representative field. p27KIP and EGFR immunostaining densities relative to tumor areas, which were confirmed by counterstaining (H&E and positive marker; p53 for DU145 and PSA for LNCaP, respectively), were quantified using CMYK quantification. Pictures are representative fields of DU145 staining for each tumor type [original magnification, x 400 (Ki67) and x 100 (others)]. Scale bar represents 25 µm (Ki67) and 100 µm (others). *P <.05, ***P <.001.