Figure 1.

RPTP-β is downregulated in metastatic HNSCC cell lines. Total RNA and whole-cell lysates were prepared from HOKCs, cells from primary HNSCC tumors (UMSCC-17A and UMSCC-22A), and cells from metastatic HNSCC tumors of the same patients (UMSCC-17B and UMSCC-22B). RPTP-β mRNA and internal control housekeeping gene 36B4 were quantified by real-time reverse transcription-PCR. RPTP-β mRNA was not detectable in cells from secondary metastatic tumors. Inset shows representative Western blot of RPTP-β protein, with β-actin used as loading control. N = 3.6, *P < .05 versus HOKCs.