Figure 8.
The kinetics of uptake of MAB1/28 and Fab1 fragments, and effect of pertussis toxin. Time course of pixel intensity in cell surface stain (A) and pixel intensity in cytoplasmic spots per cytoplasmic area (B) for MAB1/28 (22 nmol·L−1)- and Fab1 fragments (33 nmol·L−1)- induced receptor internalization in the stable CHO mGlu7 expressing cells. The curve is representative of two independent experiments shown as mean ± SDM, each experiment carried out in triplicate. The effect of PTX pre-incubation on kinetic of MAB1/28 and Fab1 receptor-binding and internalization of mGlu7 is also shown.