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. 2012 Nov 26;199(5):831–847. doi: 10.1083/jcb.201209044

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© 2012 Huang et al.

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Figure 7. — Mid1p-dependent and Cdc15p-dependent pathways are dispensable for actin filament assembly. (A) mid1-18, cdc15-140, and mid1-18 cdc15-140 cells expressing LAGFP and mCh-Atb2p were shifted to 36°C and imaged by spinning-disk microscopy at 36°C. mid1Δ and cdc15Δ cells expressing LAGFP and mCh-Atb2p were grown at 24°C and imaged by spinning-disk microscopy at 24°C. The cdc15Δ LAGFP mCh-Atb2p cell was germinated from spores. (B) Quantitation of the mutant cells in A with actin cables. Cells with spindles were grouped into two categories: short and long spindles. A single representative image from three independent experiments is shown. In each experiment, 100 cells were counted for each bar. Dashed line indicates the cell boundary. Bars, 5 µm.