Table 1.
SNP | Primer | Annealing temperature | Restriction enzyme | Fragment lengths |
---|---|---|---|---|
rs17563 | Forward 5′CACCATTCATTGCCCAAC3′ | 56°C | HphI | T Allele: 195pb + 229pb |
Reverse 5′AGTTTGGCTGCTTCTCCC3′ | C Allele: 424pb |
SNP | Primer | Annealing temperature | Restriction enzyme | Fragment lengths |
---|---|---|---|---|
rs17563 | Forward 5′CACCATTCATTGCCCAAC3′ | 56°C | HphI | T Allele: 195pb + 229pb |
Reverse 5′AGTTTGGCTGCTTCTCCC3′ | C Allele: 424pb |