Table 1. Cellular distribution of ENaC subunits in the choroid plexus, cortex, SON, PVN and SFO of Nedd 4-2 −/− mice and W/T controls.

Choroid Plexus
ENaC Subunits	Cytoplasm		Plasma Membrane
	W/T	−/−	W/T	−/−
α ENaC	3.9 ± 0.4	4.7 ± 0.6	2.3 ± 0.2	2.9 ± 0.2*
β	1.3 ± 0.4	1.5 ± 0.2	2.6 ± 0.4	5.4 ± 0.9**
γ	2.1 ± 0.4	4.3 ± 0.7*	1.4 ± 0.4	2.8 ± 0.2**
Pyramidal Cortex
α	4.0 ± 0.2	6.1 ± 0.2*	2.7 ± 0.4	5.4 ± 0.9**
β	2.6 ± 0.4	4.9 ± 0.9*	2.7 ± 0.6	4.4 ± 0.6*
γ	4.0 ± 0.6	5.1 ± 0.5*	2.6 ± 0.4	4.3 ± 0.5**
SON
α	2.2 ± 0.6	3.0 ± 1.2	1.8 ± 0.4	2.1 ± 0.7
β	2.4 ± 0.4	3.4 ± 0.5	2.4 ± 0.5	4.6 ± 0.6**
γ	1.2 ± 0.2	1.6 ± 0.4	1.6 ± 0.2	1.5 ± 0.3
PVN
α	1.2 ± 0.2	1.6 ± 0.4	1.4 ± 0.2	2.0 ± 0.4
β	1.2 ± 0.2	2.7 ± 0.5*	2.3 ± 0.2	2.9 ± 0.5
γ	1.0 ± 0.04	1.2 ± 0.2	1.1 ± 0.1	1.0 ± 0.04
SFO
α	4.9 ± 1.2	4.7 ± 1.1	4.2 ± 0.9	3.3 ± 0.9
β	3.8 ± 0.7	4.7 ± 0.8	3.4 ± 0.8	4.3 ± 0.8
γ	2.9 ± 0.6	2.5 ± 0.6	2.2 ± 0.4	2.5 ± 0.6

Values are mean ± SEM (n = 7/group for choroid plexus and cortex; n = 5/group for the 3 nuclei).

Units for cytoplasmic and membranous distribution are arbitrary (see Methods).

^*p < 0.05;

^**p < 0.01 −/− versus W/T