Table 1. Primer sequences and PCR conditions for amplification.
| Targets | Primer sequences (5′- 3′) | Annealing temperature (°C) |
|---|---|---|
| Dnmt1 |
CCAAGCTCCGGACCCTGGATGTGT |
64 |
| |
CGAGGCCGGTAGTAGTCACAGTAG |
|
| Dnmt3a |
GCACCTATGGGCTGCTGCGAAGACG |
64 |
| |
CTGCCTCCAATCACCAGGTCGAATG |
|
| Dnmt3b |
GTCTGCACACCAGAGACCAGAG |
64 |
| |
TCAGAGCCATTCCCATCATCTAC |
|
| Bmal1 |
ATGCAGAACACCAAGGAAGG |
64 |
| |
CCATCCTTAGCACGGTGAGT |
|
| Clock |
CAAAATGTCACGAGCACTTAATGC |
64 |
| |
ATATCCACTGCTGGCCTTTGG |
|
| Per1 |
ACAGCAGCCACGGTTCTC |
64 |
| |
GCTGCCACAGTCCACACA |
|
| Per2 |
CAACACAGACGACAGATCA |
64 |
| |
TCCTGGTCCTCCTTCAACAC |
|
| Cry1 |
CTCGGGTGAGGAGGTTTTCTT |
64 |
| |
GACTTCCTCTACCGAGAGCTTCAA |
|
| 18S rRNA |
TACCACATCCAAGGAAGGCAG |
64 |
| TGCCCTCCAATGGATCCTC |