Table 1. Crystallization conditions of the AKR screen.
Precipitant | Buffer, pH | Additive | |
---|---|---|---|
1 | 20% PEG 4000 | 0.1M sodium citrate pH 5.6 | 0.1M lithium sulfate |
2 | 20% PEG 4000 | 0.1M sodium citrate pH 5.6 | 0.2M sodium citrate |
3 | 23% PEG 4000 | 0.1M sodium citrate pH 5.6 | 0.2M ammonium acetate |
4 | 23% PEG 4000 | 0.1M sodium citrate pH 5.6 | 0.2M sodium acetate |
5 | 26% PEG 4000 | 0.1M sodium citrate pH 5.6 | 0.2M ammonium sulfate |
6 | 26% PEG 4000 | 0.1M sodium citrate pH 5.6 | 0.2M sodium acetate |
7 | 29% PEG 4000 | 0.1M sodium citrate pH 5.6 | 0.2M ammonium acetate |
8 | 29% PEG 4000 | 0.1M sodium citrate pH 5.6 | 0.2M ammonium sulfate |
9 | 20% PEG 4000 | 0.1M MES pH 6.4 | 0.2M ammonium acetate |
10 | 20% PEG 4000 | 0.1M MES pH 6.4 | 0.2M ammonium sulfate |
11 | 23% PEG 4000 | 0.1M MES pH 6.4 | 0.2M sodium citrate |
12 | 23% PEG 4000 | 0.1M MES pH 6.4 | 0.2M ammonium sulfate |
13 | 26% PEG 4000 | 0.1M MES pH 6.4 | 0.1M lithium sulfate |
14 | 26% PEG 4000 | 0.1M MES pH 6.4 | 0.2M ammonium acetate |
15 | 29% PEG 4000 | 0.1M MES pH 6.4 | 0.1M lithium sulfate |
16 | 29% PEG 4000 | 0.1M MES pH 6.4 | 0.2M sodium acetate |
17 | 20% PEG 4000 | 0.1M HEPES pH 7.5 | 0.2M ammonium sulfate |
18 | 20% PEG 4000 | 0.1M HEPES pH 7.5 | 0.2M sodium acetate |
19 | 23% PEG 4000 | 0.1M HEPES pH 7.5 | 0.1M lithium sulfate |
20 | 23% PEG 4000 | 0.1M HEPES pH 7.5 | 0.2M sodium acetate |
21 | 26% PEG 4000 | 0.1M HEPES pH 7.5 | 0.2M ammonium acetate |
22 | 26% PEG 4000 | 0.1M HEPES pH 7.5 | 0.2M sodium citrate |
23 | 29% PEG 4000 | 0.1M HEPES pH 7.5 | 0.1M lithium sulfate |
24 | 29% PEG 4000 | 0.1M HEPES pH 7.5 | 0.2M sodium citrate |