Figure 3.

Alignment of YjgF-family members with RutC. The amino-acid sequences of the YjgF-family members assayed in this study were aligned using ClustalW (Thompson et al., 1994 ▶) and manually edited using BioEdit (Hall, 1999 ▶). The numbering of the residues is based on the E. coli RutC sequence. Residues that are 100% conserved are highlighted; residues that are involved in active-site formation are marked with a dot. The secondary structure was calculated using PROMOTIF (Hutchinson & Thornton, 1996 ▶) on the basis of the RutC structure. Red and blue helices represent α-helices and 3₁₀-helices, respectively.