Values in parentheses are for the outer shell.

Mutant	N2D	K3N	K21Q	L27D	R30Q	K31Q	L35S
Wavelength (Å)	0.97	0.91	0.97	0.97	1.0	0.91	1.0
Crystal-to-detector distance (mm)	200	250	250	270	300	200	210
Rotation range per image (°)	0.5	0.5	0.5	0.4	1.0	0.3	0.5
Total rotation range (°)	90	140	90	120	150	120	160
Exposure time per image (s)	20	30	30	60	60	10	20
Space group	P41212	P41212	P41212	P41212	P212121	P41212	P41212
Unit-cell parameters
a (Å)	58.3	64.5	58.0	64.9	58.6	58.2	65.8
b (Å)	58.3	64.5	58.0	64.9	76.0	58.2	65.8
c (Å)	101.7	101.2	101.2	100.9	100.6	102.2	101.3
α = γ (°)	90	90	90	90	90	90	90
β	90	90	90	90	90	90	90
Resolution range (Å)	30–1.65 (1.70–1.65)	20–2.70 (2.77–2.70)	30–2.30 (2.36–2.30)	30–2.81 (2.88–2.81)	20–3.00 (3.08–3.00)	20–1.80 (1.85–1.80)	20–2.00 (2.05–2.00)
Total No. of reflections	99921	67814	41115	46757	43544	159455	191753
No. of unique reflections	21068	6311	8082	5609	9332	16817	15619
Completeness† (%)	97.4 (78.9)	99.5 (100)	99.2 (97.6)	98.8 (98.0)	98.4 (95.5)	98.7 (99.8)	99.8 (98.2)
Multiplicity	4.7 (2.0)	10.7 (11.2)	5.1 (4.7)	8.3 (7.9)	4.7 (4.6)	9.5 (9.2)	12.3 (9.7)
〈I/σ(I)〉	15.2 (1.5)	14.5 (3.1)	11.1 (2.2)	12.7 (3.4)	9.0 (1.2)	19.0 (2.5)	19.8 (1.7)
R meas ‡	0.090 (0.703)	0.181 (0.838)	0.153 (0.846)	0.156 (0.717)	0.142 (1.885)	0.117 (0.981)	0.104 (1.574)
CC1/2 § (%)	99.8 (62.5)	99.7 (95.0)	99.5 (70.9)	99.6 (94.7)	99.7 (47.9)	99.9 (78.0)	99.9 (84.8)
Overall B factor from Wilson plot (Å2)	19	37	32	38	81	22	41
Protein monomers per asymmetric unit	1	1	1	1	2	1	1
Solvent content (%)	57	65	57	65	67	57	66
Phaser Z-score¶	27	45	26	41	28	24	31