Figure 1. Generation of Animal Model.

The presence of sperm was used to indicate gestation day 1 (GD 1). Rat dams or pups were exposed to a liquid diet containing ethanol either during the: (i) 1st trimester equivalent  =  prenatal ethanol exposure 1 (PNEE₁); (ii) 2^nd trimester equivalent  =  prenatal ethanol exposure 2 (PNEE₂); or (iii) 3^rd trimester equivalent  =  ethanol exposure 3 (EE₃). Appropriate pair-fed and ad libitum animals were also reared. Dentate gyrus field recordings were conducted in early adulthood (postnatal day (PD) 50–70).