Table 2. F statistics and p-values for GLM analysis testing for differences in eyespot trait sizes across treatments.

			B. anynana				J. coenia
Surface	Wing	Trait	N (5E1)	N (NS1)	F	p	N (5E1)	N (NS1)	F	p
Ventral	Forewing	M1 white	NA	NA	NA	NA	82	59	2.67	0.10365
		M1 black	40	48	2.37	0.09760	82	59	0.003	0.95416
		M1 gold	40	48	0.19	0.82595	82	59	0.68	0.40793
		Cu1 white	40	49	1.32	0.27048	82	59	29.47	<0.0000 ***
		Cu1 black	40	49	3.19	0.04455 *	82	59	0.38	0.53503
		Cu1 gold	40	49	4.59	0.01192 *	82	59	2.36	0.12594
	Hindwing	M1 inner disc	NA	NA	NA	NA	82	59	13.76	0.00026 ***
		M1 gold	NA	NA	NA	NA	82	59	4.75	0.03033 *
		Cu1 black/inner	37	49	1.44	0.23993	82	59	8.97	0.00305 **
		Cu1 gold	37	49	0.98	0.37855	82	59	6.31	0.01271 *
Dorsal	Forewing	M1 white	NA	NA	NA	NA	82	59	23.13	<0.0000 ***
		M1 black	40	46	0.98	0.37734	82	59	13.10	0.00036 ***
		M1 gold	40	47	2.45	0.09086	82	59	12.97	0.00039 ***
		Cu1 white	40	48	1.62	0.20285	82	59	14.98	0.00014 ***
		Cu1 black	37	41	4.37	0.01487 *	82	59	5.07	0.02529 *
		Cu1 gold	37	41	8.37	0.00040 ***	82	59	9.72	0.00206 **
	Hindwing	M1 inner	NA	NA	NA	NA	82	59	0.43	0.50912
		M1 gold	NA	NA	NA	NA	82	59	1.49	0.22313
		Cu1 inner	NA	NA	NA	NA	82	59	3.90	0.04938 *
		Cu1 gold	NA	NA	NA	NA	82	59	7.98	0.00516 **

Individuals were injected with either 5E1 antibody or NS1 control media as larvae. All significant differences correspond to smaller trait sizes in the 5E1-injected individuals. GLM analyses were performed with sex (always significant across traits with females usually displaying a larger trait size than males, not shown); and interaction between line and sex (not significant in all analyses; not shown). *p<0.05, **p<0.01, ***p<0.001.