Table 3. F statistics and p-values for GLM analysis testing for differences in eyespot trait sizes across treatments using wing size as a covariate.

			B. anynana				J. coenia
Surface	Wing	Trait	N (5E1)	N (NS1)	F	p	N (5E1)	N (NS1)	F	p
Ventral	Forewing	M1 white	NA	NA	NA	NA	82	59	1.381	0.241
		M1 black	40	48	0.250	0.618	82	59	0.657	0.419
		M1 gold	40	48	0.490	0.486	82	59	1.360	0.245
		Cu1 white	40	49	0.066	0.797	82	59	0.014	0.905
		Cu1 black	40	49	0.115	0.735	82	59	5.455	0.020 *
		Cu1 gold	40	49	0.097	0.757	82	59	4.152	0.031 *
	Hindwing	M1 inner	NA	NA	NA	NA	82	59	1.558	0.213
		M1 gold	NA	NA	NA	NA	82	59	0.001	0.989
		Cu1 black/inner	37	49	0.063	0.803	82	59	1.023	0.313
		Cu1 gold	37	49	0.125	0.725	82	59	1.482	0.225
Dorsal	Forewing	M1 white	NA	NA	NA	NA	82	59	0.418	0.519
		M1 black	40	46	0.049	0.826	82	59	0.612	0.431
		M1 gold	40	47	0.469	0.496	82	59	0.788	0.376
		Cu1 white	40	48	2.961	0.090	82	59	2.123	0.147
		Cu1 black	37	41	0.071	0.790	82	59	5.308	0.022 *
		Cu1 gold	37	41	0.386	0.537	82	59	5.337	0.0218 *
	Hindwing	M1 inner	NA	NA	NA	NA	82	59	0.276	0.600
		M1 gold	NA	NA	NA	NA	82	59	0.259	0.611
		Cu1 inner	NA	NA	NA	NA	82	59	0.094	0.797
		Cu1 gold	NA	NA	NA	NA	82	59	0.318	0.573

Individuals were injected with either 5E1 antibody or NS1 control media as larvae. All significant differences correspond to smaller trait sizes in the 5E1-injected individuals. GLM analyses were performed with sex (always significant across traits with females usually displaying a larger trait size than males; not shown); interaction between line and sex (not significant in all analyses; not shown); and wing size as a covariate. *p<0.05.