Table 1. Δp52&p36 merozoites are capable of inducing a blood-stage infection.
Experiment no. | No. Asexual positive/No.injected (mean±sd pre-patency) | |
Δp52&p36 | WT | |
1 | 4/4 (6±0 days) | 2/2 (3±0 days) |
2 | 5/5 (5.8±0.4 days) | 3/3 (2±0 days) |
Huh-7 cells were infected with Δp52&p36 and WT parasites and cultured for 65 hours. After 65 hours, culture supernatant containing merozoites was collected and injected i.v in C57BL/6 mice. Regular Giemsa staining was performed in all groups, 2–14 days post i.v injection in mice, to control for asexual parasites.