Table 3.
Relative transcript abundance of sucrolytic and related regulatory genes
| Gene1 | Treatment | dppd | Leaf | Stem | Root | Panicle |
|---|---|---|---|---|---|---|
|
AhSuS-1 |
HD |
1 |
0.18 ± 0.00 |
1.50 ± 0.20 |
2.09 ± 0.10 |
— |
| 5 |
1.38 ± 0.05 |
0.87 ± 0.21 |
0.83 ± 0.08 |
— |
||
| 30 |
3.01 ± 0.24 |
2.15 ± 0.40 |
1.03 ± 0.19 |
2.15 ± 0.08 |
||
| MD |
1 |
0.63 ± 0.06 |
NE |
0.95 ± 0.16 |
— |
|
| 5 |
0.87 ± 0.07 |
0.84 ± 0.07 |
0.53 ± 0.08 |
— |
||
| 30 |
1.23 ± 0.27 |
1.06 ± 0.30 |
0.55 ± 0.01 |
1.47 ± 0.35 |
||
|
AhSuS-2 |
HD |
1 |
0.36 ± 0.03 |
2.10 ± 0.10 |
1.64 ± 0.21 |
— |
| 5 |
1.21 ± 0.02 |
0.77 ± 0.09 |
1.52 ± 0.03 |
— |
||
| 30 |
2.62 ± 0.07 |
1.58 ± 0.14 |
1.77 ± 0.25 |
1.91 ± 0.05 |
||
| MD |
1 |
0.76 ± 0.04 |
2.36 ± 0.41 |
1.19 ± 0.10 |
— |
|
| 5 |
0.86 ± 0.08 |
0.66 ± 0.01 |
0.86 ± 0.23 |
— |
||
| 30 |
1.13 ± 0.08 |
2.00 ± 0.21 |
1.10 ± 0.06 |
1.07 ± 0.04 |
||
|
AhVI-1 |
HD |
1 |
2.19 ± 0.18 |
3.00 ± 0.90 |
1.37 ± 0.13 |
— |
| 5 |
3.74 ± 0.00 |
6.58 ± 0.26 |
2.49 ± 0.05 |
— |
||
| 30 |
0.91 ± 0.25 |
1.12 ± 0.15 |
1.20 ± 0.18 |
1.38 ±0.02 |
||
| MD |
1 |
0.82 ± 0.01 |
1.25 ± 0.06 |
1.96 ± 0.14 |
— |
|
| 5 |
2.57 ± 0.06 |
2.71 ± 0.76 |
1.30 ± 0.43 |
— |
||
| 30 |
1.24 ± 0.04 |
1.11 ± 0.13 |
0.77 ± 0.06 |
1.65 ± 0.04 |
||
|
AhInvI-4(VI inhibitor) |
HD |
1 |
0.40 ± 0.17 |
1.00 ± 0.30 |
1.54 ± 0.11 |
— |
| 5 |
1.00 ± 0.01 |
0.89 ± 0.34 |
0.78 ± 0.22 |
— |
||
| 30 |
1.77 ± 0.29 |
0.60 ± 0.06 |
0.72 ± 0.18 |
1.30 ± 0.22 |
||
| MD |
1 |
0.68 ± 0.40 |
1.12 ± 0.39 |
0.86 ± 0.09 |
— |
|
| 5 |
0.96 ± 0.06 |
0.61 ± 0.34 |
0.61 ± 0.16 |
— |
||
| 30 |
2.89 ± 0.53 |
1.86 ± 0.04 |
0.98 ± 0.10 |
0.76 ± 0.09 |
||
|
Ah-γVPE |
HD |
1 |
4.40 ± 0.54 |
0.70 ± 0.10 |
1.41 ± 0.19 |
— |
| 5 |
0.10 ± 0.01 |
1.91 ± 0.25 |
2.74 ± 0.52 |
— |
||
| 30 |
5.83 ± 0.92 |
0.98 ± 0.30 |
1.61 ± 0.13 |
2.09 ± 0.11 |
||
| MD |
1 |
0.49 ± 0.20 |
0.59 ± 0.27 |
1.15 ± 0.11 |
— |
|
| 5 |
0.35 ± 0.04 |
0.39 ± 0.12 |
0.13 ± 0.01 |
— |
||
| 30 |
3.59 ± 0.01 |
1.10 ± 0.55 |
1.39 ± 0.14 |
1.76 ± 0.09 |
||
|
AhInvI-2(CWI inhibitor) |
HD |
1 |
0.23 ± 0.02 |
1.40 ± 0.20 |
1.51 ± 0.23 |
— |
| 5 |
1.59 ± 0.14 |
0.85 ± 0.06 |
0.75 ± 0.10 |
— |
||
| 30 |
1.94 ± 0.28 |
1.12 ± 0.02 |
0.97 ± 0.03 |
1.39 ± 0.16 |
||
| MD |
1 |
0.35 ± 0.19 |
2.45 ± 1.69 |
1.29 ± 0.16 |
— |
|
| 5 |
1.30 ± 0.00 |
0.56 ± 0.04 |
1.00 ± 0.06 |
— |
||
| 30 |
0.88 ± 0.01 |
2.56 ± 0.15 |
0.94 ± 0.16 |
1.26 ± 0.10 |
||
|
AhInvI-1(CWI inhibitor) |
HD |
1 |
0.20 ± 0.03 |
2NE |
0.29 ± 0.06 |
— |
| 5 |
0.53 ± 0.09 |
0.96 ± 0.01 |
0.98 ± 0.00 |
|
||
| 30 |
1.03 ± 0.13 |
0.91 ± 0.16 |
0.70 ± 0.03 |
1.05 ± 0.13 |
||
| MD |
1 |
0.70 ± 0.05 |
NE |
1.22 ± 0.16 |
— |
|
| 5 |
0.63 ± 0.04 |
1.19 ± 0.51 |
1.86 ± 0.54 |
— |
||
| 30 |
0.94 ± 0.22 |
2.48 ± 0.23 |
2.08 ± 0.19 |
0.93 ± 0.15 |
||
|
AhA/NI-1 |
HD |
1 |
0.09 ± 0.20 |
0.79 ± 0.26 |
NE |
— |
| |
|
5 |
0.94 ± 0.37 |
3.59 ± 0.31 |
NE |
— |
| |
|
30 |
NE |
1.38 ± 0.16 |
NE |
NE |
| |
MD |
1 |
0.11 ± 0.20 |
1.64 ± 0.06 |
NE |
— |
| |
|
5 |
0.22 ± 0.47 |
6.10 ± 0.22 |
NE |
— |
| |
|
30 |
NE |
0.80 ± 0.17 |
NE |
NE |
|
AhSNRK-1 |
HD |
1 |
1.87 ± 0.05 |
— |
— |
— |
| 5 |
1.29 ± 0.38 |
— |
— |
— |
||
| 30 |
0.40 ± 0.27 |
— |
— |
— |
||
| MD |
1 |
0.18 ± 0.16 |
— |
— |
— |
|
| 5 |
4.70 ± 0.50 |
— |
— |
— |
||
| 30 | 3.87 ± 0.27 | — | — | — |
Expression was measured at different days post partial defoliation (dppd) by insect (HD) or mechanical (MD) damage in different plant tissues of Amaranthus cruentus. Transcript levels were measured by qRT-PCR. Values represent means ± SE of a representative experiment with three replicates. They indicate the fold-change in gene expression levels present in tissues of defoliated plants relative to those in tissues of intact control plants. A given gene was considered induced when its relative expression values was ≥ 1.5, whereas it was considered repressed when its relative expression values was ≤ 0.5. These are shown in bold text and bold italics, respectively.
1The expression of the neutral invertase AhA/NI-2 and sucrose phosphate synthase (AhSPS) genes was not detected in any of the tissues examined.
2NE = Not Expressed.