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Forward and reverse primer sequences used for qRT-PCR measurements synthesized by Integrated DNA Technologies (Coralville, IA)
| Gene | Forward primer 5′→ 3′ | Reverse primer 5′→ 3′ |
|---|---|---|
| PGC-1α |
ACCAAACCCACAGAGAACAG |
GGGTCAGAGGAAGAGATAAAGTTG |
| GLUT4 |
AAGAATCCCTGCAGCCTGGTAGAA |
CCACGGCCAAACCACAACACATAA |
| Irisin |
AGGTGCTTTACCGCTGTACCTTCA |
AGAGAGGGCCAGATGTTTGTTGGA |
| TBP | CACGAACCACGGCACTGATT | TTTTCTTGCTGCCAGTCTGGAC |
