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. 2012 Dec 5;7(12):e51089. doi: 10.1371/journal.pone.0051089

Figure 3. In situ ELISA assay validation.

Figure 3

(A) Correlation between the in situ ELISA and the foci-forming assay, where Huh7.5 was infected with DENV-1, DENV-2, DENV-3 and DENV-4 at a range of MOI (4–0.01). After 72 hours of infection the supernatant was used for the foci-forming assay and the cells submitted to the ELISA assay. Data from one representative experiment, analyzed using Pearson correlation test. (B) Comparison of the DRC and the IC50 for IFN-α 2A and DENV-4 infection, obtained with the in situ ELISA, foci-forming assay and a commercial NS1 antigen capture ELISA assay. Mean ± SD of three independent experiments, analyzed by sigmoidal dose-response curve (variable slope), the dashed line represents the detection limit of the foci-forming assay.