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. 2012 Dec 5;7(12):e50927. doi: 10.1371/journal.pone.0050927

Figure 1. Structural domain arrangements of wild-type Sis1 and mutants.

Figure 1

(A) Sis1 contains the J-domain and the G/F region located in the N-terminal region (121 amino acids residues – red box). In the central portion, between the G/F domain and the C-terminus, Sis1 has a G/M-rich (Glycine/Methionine-rich – green box) domain and Ydj1 has a zinc-finger-like region (ZFLR; yellow box). The C-terminal domains (CTD) I and II follow (orange and blue boxes, respectively). In Sis1_Δ124–174 the G/M region is absent and in Sis1_Δ121–257 both the G/M region and the CTDII are deleted. The chimera SYS is formed by the J-domain and the CTDII from Sis1 and the middle region from Ydj1. Ydj1 is shown for comparison. PDB accession numbers: 2O37 (red, residues 1–81), 1C3G (yellow, residues 180–255; blue: 256–349) [16], 1NLT (yellow, residues 110–255 and blue 256–337) [13]. (B) Amino acid sequence alignment of Sis1, SYS and Ydj1. Red box, J-domain; Green, G/M-rich region; red, CTDI; blue, CDTII; yellow, permuted region between Ydj1 and Sis1; gray, dimerization region of Sis1.