Figure 7. NMR experiments for Sis1 and Sis1_Δ_124–174.

(A) Superimposed TROSY NMR spectra of Sis1 (black signals) and Sis1_Δ_124–174 (red signals) showing that Sis1_Δ_124–174 presents less signals, all of them in the central region of the TROSY spectrum with chemical shifts around 8.2. (B) Zoom of the 2D ¹H ¹⁵N chemical shifts of Sis1 showing the crosspeaks (marked in green) which are present in the wild-type Sis1 spectra but absent in the Sis1_Δ_124–174 spectra. The NMR experiments suggest that the deleted region of Sis1 is intrinsically disordered.