Table 1. Summary of the thermodynamic data obtained from unfolding experiments.
Thermodynamic Property | Protein | |||
Sis1 | Sis1_Δ124–174 | Sis1_Δ121–257 | SYS | |
ΔG H 2 O N-I (kcal/mol) | 7.2±0.5 | 6.2±0.2 | 4.8±0.6 | 6.0±0.8 |
ΔG H 2 O I-U (kcal/mol) | 8.0±0.1 | 6.6±0.8 | 6.3±0.3 | 5.9±0.2 |
mN-I (kcal/mol.M) | 2.1±0.2 | 2.0±0.2 | 2.4±0.3 | 3.3±0.4 |
mI-U (kcal/mol.M) | 1.7±0.2 | 1.5±0.2 | 1.5±0.6 | 1.4±0.4 |
CmN-I (M)† | 3.3±0.1 | 3.2±0.1 | 2.0±0.1 | 1.8±0.1 |
CmI-U (M)† | 4.5±0.1 | 4.5±0.2 | 4.3±0.1 | 4.3±0.1 |
CmAUC (M)‡ | 3.4±0.1 | 3.5±0.1 | 2.1±0.2 | 1.4±0.1 |
CmSEC-MALLS (M)‡ | 3.5±0.1 | 3.4±0.1 | 2.4±0.2 | 1.9±0.1 |
Tm1 (°C) | 59.0±0.2 | 59.2±0.4 | 53.8±0.2 | 45.1±0.7 |
Tm2 (°C) | 67.7±0.6 | 67.3±0.2 | 65.6±0.5 | 63.7±0.4 |
ΔH1cal (kcal/mol) | 154.0±0.2 | 149.3±0.5 | 70.3±0.5 | 84.3±0.8 |
ΔH2cal (kcal/mol) | 29.5±0.5 | 29.3±0.2 | 48.2±0.6 | 59±0.1 |
ΔCp1 (kcal/mol/K)* | 4.0±0.4 | 5.1±0.6 | 3.1±0.4 | 3.8±0.1 |
ΔCp2 (kcal/mol/K)* | 1.3±0.4 | 1.4±0.2 | 1.4±0.5 | 1.7±0.7 |
TmCD (°C) | 60.1±0.5 | 61.4±0.5 | 56.4±0.5 | 59.3±0.5 |