Table 1. Summary of the thermodynamic data obtained from unfolding experiments.

Thermodynamic Property	Protein
	Sis1	Sis1_Δ124–174	Sis1_Δ121–257	SYS
ΔG H 2 O N-I (kcal/mol)	7.2±0.5	6.2±0.2	4.8±0.6	6.0±0.8
ΔG H 2 O I-U (kcal/mol)	8.0±0.1	6.6±0.8	6.3±0.3	5.9±0.2
mN-I (kcal/mol.M)	2.1±0.2	2.0±0.2	2.4±0.3	3.3±0.4
mI-U (kcal/mol.M)	1.7±0.2	1.5±0.2	1.5±0.6	1.4±0.4
CmN-I (M)†	3.3±0.1	3.2±0.1	2.0±0.1	1.8±0.1
CmI-U (M)†	4.5±0.1	4.5±0.2	4.3±0.1	4.3±0.1
CmAUC (M)‡	3.4±0.1	3.5±0.1	2.1±0.2	1.4±0.1
CmSEC-MALLS (M)‡	3.5±0.1	3.4±0.1	2.4±0.2	1.9±0.1
Tm1 (°C)	59.0±0.2	59.2±0.4	53.8±0.2	45.1±0.7
Tm2 (°C)	67.7±0.6	67.3±0.2	65.6±0.5	63.7±0.4
ΔH1cal (kcal/mol)	154.0±0.2	149.3±0.5	70.3±0.5	84.3±0.8
ΔH2cal (kcal/mol)	29.5±0.5	29.3±0.2	48.2±0.6	59±0.1
ΔCp1 (kcal/mol/K)*	4.0±0.4	5.1±0.6	3.1±0.4	3.8±0.1
ΔCp2 (kcal/mol/K)*	1.3±0.4	1.4±0.2	1.4±0.5	1.7±0.7
TmCD (°C)	60.1±0.5	61.4±0.5	56.4±0.5	59.3±0.5

^*Data estimated from the Kirchoff plot by application of Eq. 3 (Fig. 3).

^†determined from the ΔG^w_N-I/m_N-I and ΔG^w_I-U/m_I-U ratio;

^‡ Cm_AUC and Cm_SEC-MALLS are the urea concentrations of the midpoint of the s ⁰ _20,w and MM transition, respectively (Fig. 5).