Figure 2. Meth causes a reduction of splenic leukocyte subsets.

C57BL/6 mice were treated with vehicle (control) or ramped dosage of methamphetamine (meth – see methods) for 14 days. At day 14, mice were sacrificed and splenic leukocyte subsets were analyzed by flow cytometry. A. Meth treatment did not alter total splenocyte number. B. Meth causes a significant reduction in total natural killer (NK) cells (NKp46⁺, CD3⁻, B220⁻, Gr-1⁻, MHC II⁻). C. Gr-1^high monocytes (CD11b⁺, Gr-1^high, MHC II⁻, CD3⁻, B220⁻) were reduced after meth treatment. D. Meth treatment reduces dendritic cells (DCs) defined as CD11c⁺, MHC II⁺, B220⁻, Gr-1⁻ events. Dead cells and doublets were removed prior to analysis. **P<0.01 calculated by Mann-Whitney U Test. Bars represent mean. Data are from 2 experiments of 5 animals per treatment group per experiment.