Figure 6. Meth causes reduced proportions of alternatively spliced CD4 T cells among the splenic CD62L^high compartment.

Meth and control mice were analyzed for surface expression of CD45RB and CD44 on the CD62L^high subset. CD45RB expression is downregulated upon antigen experience and can be used to gauge activation levels of CD4 T cells. A. CD45RB and CD44 on CD62L^high splenic CD4 T cells. B–D. Meth-treated animals had a higher proportion of CD45RB^high, CD44^low CD4 T cells compared to controls. Meth-treated animals also exhibited lower proportions of CD45RB^low, CD44^low and CD45RB^low, CD44^highsubsets compared to controls. ***p<0.001 calculated by Mann-Whitney U Test. Bars represent mean. Data are from 2 experiments of 5 animals per treatment group per experiment.