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View full-text article in PMC

. 2012 Dec 5;7(12):e49897. doi: 10.1371/journal.pone.0049897

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© 2012 Harms et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

We examined the expression of the costimulatory markers CD150 and CD226 by splenic CD4 T cells after meth treatment. A. Representative gating and histogram showing selection of CD226⁺ CD4 T cells and increased expression of CD150 by meth treated animals compared to control. Red represents a meth-treated animal, black is vehicle-treated animal, and grey is the isotype control. B. CD226⁺ CD4 T cells from meth treated animals express higher levels of CD150 than those from control animals. Protein expression was calculated using mean fluorescence intensity (MFI). ***p<0.001 calculated by Mann-Whitney U Test. Error bars represent SEM. Data are from 2 experiments of 5 animals per treatment group per experiment.