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. 2012 Dec 5;7(12):e50265. doi: 10.1371/journal.pone.0050265

Figure 4. Prolonged viability of encapsulated islets in vitro.

Figure 4

(A) Staining of viable (green) versus dead (red) cells in cultures of naked islets (CTR), pegylated islets (PEG), or pegylated plus empty-nanoparticle islets (Nano) at 1 d, 7 d, 14 d, and 21 d. (B) Percentages of viable cells in the different groups during culture. (C) Insulin staining in naked (CTR) and PEG-Nano-coated islets at 2 d and 14 d culture: more insulin positive cells were observed in islets encapsulated with nanoparticles (lower panels) compared to naked islets (upper panels). At least 10 islets were included in each group. Red represents insulin staining, blue staining (DAPI) represents nuclear staining in all cells. * p<0.05 and ** p<0.01.