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. 2012 Dec 5;7(12):e46888. doi: 10.1371/journal.pone.0046888

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© 2012 Wang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A–C) Typical results for the MTA1 staining pattern determined by immunohistochemistry are shown in normal prostate tissue (A), localized prostate cancer tissue (B), and metastatic prostate cancer tissue (C). The results in B and C showed MTA1 expression in both nuclei and cytoplasm. The original magnification for A–C is 200 fold and detailed with enlarged view is 400 fold. (D) A quantitative RT-PCR analysis of the MTA1 RNA levels in 2B4 and 1E8 cells (top). An asterisk (*) indicates a statistically significant difference (p<0.05) compared to the 2B4 cells. (E) The protein expression levels of MTA1 in 2B4 and 1E8 cells were analyzed by Western blotting using specific antibodies, and the results were quantified (top). An asterisk (*) indicates a statistically significant difference (p<0.05) compared to the 2B4 cells. The experiments were repeated three times.