Figure 1. Components of the Vid30c are required for Hxt3 turnover.

BY4742 (WT) and the indicated Vid30c mutants expressing HXT3-GFP were cultured in glucose media as described in the methods (time 0). Following a switch to ethanol media, samples were collected at the indicated times and analyzed by (A) fluorescence microscopy, and (B) Western analysis with anti-GFP antibodies. Identical blots were also probed with anti-ADH antibody as a loading control.