Figure 9. The turnover of Hxt3 is dependent on Rsp5 and Art8.

(A and B) BY4742 (WT) and rsp5-1, and (C) BY4742 (WT), art3Δ, art4Δ, art6Δ and art8Δ expressing HXT3-GFP were cultured in glucose media as described in the methods (time 0). Following a switch to ethanol media, samples were collected at the indicated times and analyzed by (A) fluorescence microscopy, and (B and C) Western analysis with anti-GFP antibodies. Identical blots were also probed with anti-ADH antibody as a loading control.