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. 2012 Dec 5;7(12):e50436. doi: 10.1371/journal.pone.0050436

Figure 4. Overexpression of CARP inhibits the activation of MEK/ERK signaling by hypertrophic stimuli.

Figure 4

(A) Western blotting of heart protein extracts to examine the levels of various phosphorylated and total kinases in WT and CARP Tg mice following TAC or a sham operation. A representative Western blot (from one of three independent experiments) is shown. (B) Western blotting of protein extracts to detect various phosphorylated and total kinase levels in parental and CARP-overexpressing cardiomyocytes subjected to phenylephrine treatment or not. A representative Western blot (from one of three independent experiments) is shown. (C) Quantification of the expression of the p-MEK1/2 and p-ERK1/2 proteins shown in (B) at 30 min after commencement of PE treatment.