Figure 1. Identification of DNA-PKcs as a BPA-binding protein.

(A) The structure of BPA. (B) Preparation of BPA-immobilized beads. (C) BPA-binding proteins were purified from MCF7 cell nuclear extracts using BPA-immobilized (+) or control (−) beads. Bound proteins were eluted with SDS sample buffer. In lanes 3 and 4, BPA was added to extracts before incubation with the beads. Eluted proteins were analyzed by silver staining (C) or immunoblotting (IB) (D).