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. 2012 Dec 5;7(12):e50481. doi: 10.1371/journal.pone.0050481

Figure 2. Binding of BPA to recombinant DNA-PKcs fragments in vitro.

Figure 2

(A) Structure of DNA-PKcs and seven fragments. (B) Recombinant DNA-PKcs fragments (#1 to #7) were purified from 293T cells and incubated with BPA-immobilized (+) or control (−) beads. The input and bound proteins were immunoblotted with an anti-FLAG antibody. (C) The Kd for the binding of BPA to ΔDNA-PKcs #6 was calculated by Scatchard analysis.