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. 2012 Dec 5;7(12):e50509. doi: 10.1371/journal.pone.0050509

Figure 6. Depletion of agrin and of neuregulin contributes to defective NMJ formation in Hnf6 mutant mice.

Figure 6

A–H, NMJ-like structures obtained in vitro by co-culture of control myotubes with control (A,C,E,G) or Hnf6−/− (B,D,F,H) embryonic spinal cord slices in medium supplemented with vehicle only (A,B) or with recombinant agrin (C,D), neuregulin (E,F) or TGFß (G,H). I , J, Quantification of the length (I) and of the area (J) of the NMJ-like structures obtained with control (blue) or Hnf6−/− (red) spinal cord slices. The size and area deficits observed with Hnf6−/− slices are fully rescued by agrin and by neuregulin, but not by TGFß (n = 3). Nrg-1: neuregulin-1. Dunett’s (I) and Newman-Keuls (J) multiple comparison tests; * = p<0.05, ** = p<0.01, *** = p<0.001. Scale bar = 10 µm.