Figure 5.
Confocal microscope images of double-immunofluorescence staining of mesenteric resistant arteries (A, B, C, G, H, I) and DRG neurons (D, E, F). A, D and G: FITC-labeled TRPV4 receptor staining (green). B and E: Cy3-labeled CGRP staining (red). C and F: colocalization of TRPV4 and CGRP (yellow). H: Cy3-labeled MaxiК channels staining (red). I: colocalization of TRPV4 and MaxiК channels (yellow). Blue arrows: the colocalized areas. Negative control not shown. Scale bars, 20 μm.